IκB-beta(Phospho Ser23) Polyclonal Antibody

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Home > Product > Antibody > IκB-beta(Phospho Ser23) Polyclonal Antibody

IκB-beta(Phospho Ser23) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

NF-kappa-B inhibitor beta

Code

BT-AP10546

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Ser23. AA range:8-57

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

37771

Gene ID

Human: 4793; Mouse: 18036

Synonyms

NF-kappa-B inhibitor beta; NFKBIB; IKBB; TRIP9; NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9

Research area

Epigenetics and nuclear signaling; Immunology; Signal transduction

Target protein

NFKBIB

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 40000

Images

Immunofluorescence analysis of HeLa cells treated with TNF-a 20nM 15', using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using IkappaB-beta (Phospho-Ser23) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-IκB-β (S23) Polyclonal Antibody

Western blot analysis of lysates from HeLa cells treated with TNF-a 20ng/ml 5', using IkappaB-beta (Phospho-Ser23) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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