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Home > Product > Antibody > LC3B Polyclonal Antibody

LC3B Polyclonal Antibody

LC3B Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Microtubule-associated proteins 1A/1B light chain 3B

Code

BT-AP10857

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

Recombinant Protein of MAP LC3β

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for 1 year

Background

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Uniprot accession

Gene ID

Human: 81631

Synonyms

Microtubule-associated proteins 1A/1B light chain 3B; MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta

Research area

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal transduction

Target protein

MAP1LC3B

Recommended dilution

Application Dilution
WB 1: 1000 - 1: 2000
IHC 1: 200 - 500
IF 1: 200

Images

Immunofluorescence analysis of Hela cell. Cleaved-PARP-1 (D214) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). LC3B Polyclonal Antibody(Green) was diluted at 1:200(4°C overnight).
Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat BrainTissue using MAP LC3β Polyclonal Antibody.
Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Monoclonal Antibody(2B8) antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using MAP LC3β Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Protocols

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Product size

Price

  • 20ul

    $108.00

  • 50ul

    $180.00

  • 100ul

    $275.00

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Lead time: Within one week

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