Immunofluorescence analysis of Hela cell. Cleaved-PARP-1 (D214) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). LC3B Polyclonal Antibody(Green) was diluted at 1:200(4°C overnight).
Immunofluorescence analysis of Mouse-lung tissue. 1,LC3B Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,LC3B Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat BrainTissue using MAP LC3β Polyclonal Antibody.
Western blot analysis of lysates from 1) Hela, 2) 3T3, 3) Rat Brain Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C overnight, secondary antibody was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Monoclonal Antibody(2B8) antibody was diluted at 1:5000 as loading control, 4°C overnight,secondary antibody was diluted at 1:10000, 37°C 1hour.
Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Brain Tissue using MAP LC3β Polyclonal Antibody. Secondary antibody was diluted at 1:20000
