NBK(Phospho Thr33) Polyclonal Antibody

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Home > Product > Antibody > NBK(Phospho Thr33) Polyclonal Antibody

NBK(Phospho Thr33) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Rat, Mouse

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Bcl-2-interacting killer

Code

BT-AP11711

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human BIK around the phosphorylation site of Thr33. AA range:18-67

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

Uniprot accession

Human:;Mouse:;

Molecular weight

18016

Gene ID

Human: 638

Synonyms

Bcl-2-interacting killer; BIK; NBK; Bcl-2-interacting killer; Apoptosis inducer NBK; BIP1; BP4

Research area

Cancer; Cell biology

Target protein

BIK

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 10000

Images

Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using BIK (Phospho-Thr33) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells, using BIK (Phospho-Thr33) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

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Lead time: Within one week

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