Net(Phospho Ser357) Polyclonal Antibody

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Blood coagulation
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Home > Product > Antibody > Net(Phospho Ser357) Polyclonal Antibody

Net(Phospho Ser357) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Monkey

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

ETS domain-containing protein Elk-3

Code

BT-AP11812

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Elk3 around the phosphorylation site of Ser357. AA range:323-372

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a member of the ETS-domain transcription factor family and the ternary complex factor (TCF) subfamily. Proteins in this subfamily regulate transcription when recruited by serum response factor to bind to serum response elements. This protein is activated by signal-induced phosphorylation; studies in rodents suggest that it is a transcriptional inhibitor in the absence of Ras, but activates transcription when Ras is present. Alternate splicing results in multiple transcript variants.

Uniprot accession

Human:;Mouse:;

Molecular weight

44240

Gene ID

Human: 2004; Mouse: 13713

Synonyms

ETS domain-containing protein Elk-3; ELK3; NET; SAP2; ETS domain-containing protein Elk-3; ETS-related protein ERP; ETS-related protein NET; Serum response factor accessory protein 2; SAP-2; SRF accessory protein 2

Research area

Cardiovascular; Developmental biology; Epigenetics and nuclear signaling; Stem cells

Target protein

ELK3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunofluorescence analysis of HeLa cells, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using Elk3 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of COLO cells using Phospho-Net (S357) Polyclonal Antibody

Western blot analysis of lysates from COLO205 cells treated with serum 20% 15', using Elk3 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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