p70 S6 Kinase alpha(Phospho Thr229) Polyclonal Antibody

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Home > Product > Antibody > p70 S6 Kinase alpha(Phospho Thr229) Polyclonal Antibody

p70 S6 Kinase alpha(Phospho Thr229) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Ribosomal protein S6 kinase beta-1

Code

BT-AP12695

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr229. AA range:195-244

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

59140

Gene ID

Human: 6198; Mouse: 72508; Rat: 83840

Synonyms

Ribosomal protein S6 kinase beta-1; RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinas

Research area

Cell biology; Epigenetics and nuclear signaling; Metabolism; Signal transduction

Target protein

RPS6KB1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 5000

Images

Immunofluorescence analysis of Hela cell. p70 S6 kinase α (phospho Thr229) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). Caspase-8 Monoclonal Antibody(2G12)(Green) was diluted at 1:200(4°C overnight).

Immunohistochemistry analysis of paraffin-embedded human brain, using p70 S6 Kinase (Phospho-Thr229) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of various cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500

Western Blot analysis of LOVO cells using Phospho-p70 S6 kinase α (T229) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from Jurkat cells, using p70 S6 Kinase (Phospho-Thr229) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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