p70 S6 Kinase alpha Polyclonal Antibody

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Home > Product > Antibody > p70 S6 Kinase alpha Polyclonal Antibody

p70 S6 Kinase alpha Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IF, ICC, IHC-p

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF, ICC, IHC-p

Product overview

Full product name

Ribosomal protein S6 kinase beta-1

Code

BT-AP12706

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

Recombinant Protein of p70 S6 kinase α

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation

PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Storage

-20°C for 1 year

Background

This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.

Uniprot accession

Human:;Mouse:;Rat:;

Gene ID

Human: 6198

Synonyms

Ribosomal protein S6 kinase beta-1; RPS6KB1; STK14A; Ribosomal protein S6 kinase beta-1; S6K-beta-1; S6K1; 70 kDa ribosomal protein S6 kinase 1; P70S6K1; p70-S6K 1; Ribosomal protein S6 kinase I; Serine/threonine-protein kinase 14A; p70 ribosomal S6 kinase alpha; p70 S6 kinase alpha; p70 S6K-alpha; p70 S6KA

Research area

Cell biology; Epigenetics and nuclear signaling; Metabolism; Signal transduction

Target protein

RPS6KB1

Recommended dilution

Application	Dilution
WB	1: 1000 - 1: 2000
IHC	1: 200 - 1: 500
IF	1: 200

Images

Immunofluorescence analysis of Mouse-brain tissue. 1,p70 S6 kinase α Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,p70 S6 kinase α Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,p70 S6 kinase α Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,p70 S6 kinase α Polyclonal Antibody was diluted at 1:200(4°C overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using p70 S6 kinase α Polyclonal Antibody.

Western blot analysis of 1) Hela, 2) 3T3, 3) PC12 using p70 S6 kinase α Polyclonal Antibody. Secondary antibody was diluted at 1:20000

Western blot detection of p70S6 in human breast cancer cell line MCF-7(A), T47D(B), MDA-MB-231(C) and Cal51 (D) using p70S6 rabbit polyAb (YM3426, 1:1000 diluted).Predicted band size: 70kDa.Observed band size:70kDa. Picture was kindly provided by our customer from Tianjin Medical University Cancer Institute and Hospital

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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