Smad3(Phospho Ser204) Polyclonal Antibody

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Home > Product > Antibody > Smad3(Phospho Ser204) Polyclonal Antibody

Smad3(Phospho Ser204) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ICC, ELISA

Product overview

Full product name

Mothers against decapentaplegic homolog 3

Code

BT-AP14300

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204. AA range:170-219

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis.

Uniprot accession

Human:;Mouse:;Rat:;

Molecular weight

48081

Gene ID

Human: 4088; Mouse: 17127; Rat: 25631

Synonyms

Mothers against decapentaplegic homolog 3; SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Research area

Epigenetics and nuclear signaling; Signal transduction; Metabolism; Cancer; Stem cells

Target protein

SMAD3

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
IF	1: 200 - 1: 1000
ICC	1: 200 - 1: 1000
ELISA	1: 20000

Images

Immunofluorescence analysis of Hela cell. Smad3 (phospho Ser204) Polyclonal Antibody(Red) was diluted at 1:200(4°C overnight). LC3A mouse Monoclonal Antibody(5G10)(Green) was diluted at 1:200(4°C overnight).

Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4°C overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Western Blot analysis of various cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1:500

Western Blot analysis of MCF-7 cells using Phospho-Smad3 (S204) Polyclonal Antibody diluted at 1:500

Western blot analysis of lysates from NIH/3T3 cells treated with Serum 20% 15', using Smad3 (Phospho-Ser204) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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