Stat1(Phospho Ser727) Polyclonal Antibody

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Blood coagulation
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Cell biology
Signal transduction
Developmental biology
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Epigenetics and nuclear signaling
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Home > Product > Antibody > Stat1(Phospho Ser727) Polyclonal Antibody

Stat1(Phospho Ser727) Polyclonal Antibody

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity:Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product Summary

Size: 20ul,50ul,100ul

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-p, IF, ELISA

Product overview

Full product name

Signal transducer and activator of transcription 1-alpha/beta

Code

BT-AP14527

Host

Rabbit

Isotype

IgG

Size

20ul,50ul,100ul

Clonality

Polyclonal

Immunogen

The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Ser727. AA range:694-743

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Formulation

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage

-20°C for 1 year

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.

Uniprot accession

Human:;Mouse:;

Molecular weight

87335

Gene ID

Human: 6772

Synonyms

Signal transducer and activator of transcription 1-alpha/beta; STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84

Research area

Epigenetics and nuclear signaling; Cancer; Signal transduction; Microbiology

Target protein

STAT1

Recommended dilution

Application	Dilution
WB	1: 500 - 1: 2000
IHC-p	1: 100 - 1: 300
ELISA	1: 10000

Images

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT1 (Phospho-Ser727) Antibody. The picture on the right is blocked with the phospho peptide.

Western Blot analysis of K562 293 PC-3 HepG2-UV cells using Phospho-Stat1 (S727) Polyclonal Antibody diluted at 1:1000

Western blot analysis of lysates from 293 cells, 3T3 cells treated with UV (15mins) and Jurkat cells treated with eto (25uM, 24hours), using STAT1 (Phospho-Ser727) Antibody. The lane on the right is blocked with the phospho peptide.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

20ul

$108.00
50ul

$180.00
100ul

$275.00

Add to Cart

Lead time: Within one week

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