Immunofluorescence analysis of human-uterus tissue. Actin beta Polyclonal Antibody(red) was diluted at 1: 200(4°C, overnight). Cy3 labled Secondary antibody was diluted at 1: 300 at room temperature for 50 mins. Picture B: DAPI(blue) 10 mins. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-stomach tissue. Actin beta Polyclonal Antibody(red) was diluted at 1: 200(4°C,overnight). Cy3 labled Secondary antibody was diluted at 1: 300 at room temperature for 50mins). Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of human-stomach tissue. Actin beta Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). Cy3 labled Secondary antibody was diluted at 1: 300 at room temperature for 50mins. Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. Actin beta Polyclonal Antibody(red) was diluted at 1: 200(4°C,overnight). Cy3 labled Secondary antibody was diluted at 1: 300 at room temperature for 50mins. Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of rat-lung tissue. Actin beta Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). Cy3 labled Secondary antibody was diluted at 1: 300 at room temperature for 50mins. Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. Actin beta Polyclonal Antibody was diluted at 1: 200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20 mins). Secondary antibody was diluted at 1: 200(room temperature, 30 mins). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. Actin beta Polyclonal Antibody was diluted at 1: 200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). Secondary antibody was diluted at 1: 200(room temperature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-colon tissue. Actin beta Polyclonal Antibody was diluted at 1: 200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20 min). Secondary antibody was diluted at 1: 200(room temperature, 30 min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. Actin beta Polyclonal Antibody was diluted at 1:200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20 min). Secondary antibody was diluted at 1:200(room temperature, 30 min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. Actin beta Polyclonal Antibody was diluted at 1: 200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20 min). Secondary antibody was diluted at 1: 200(room temperature, 30 min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung tissue. Actin beta Polyclonal Antibody was diluted at 1: 200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20 min). Secondary antibody was diluted at 1: 200(room temperature, 30 min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. Actin beta Polyclonal Antibody was diluted at 1: 200(4°C,overnight). Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20 min). Secondary antibody was diluted at 1: 200(room temperature, 30 min). Negative control was used by secondary antibody only.
Western Blot analysis of 1, hela 2, A549 3, HEPG2 4, Mouse-brain 5, Mouse-lung 6, Mouse-liver 7, Rat-brain 8, Rat-lung 9, Rat-liver cells using primary antibody diluted at 1: 1000(4°C overnight). Secondary antibody: Goat Anti-rabbit IgG IRDye 800( diluted at 1: 5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit (SM-005, Inventbiotech, MN, USA).
Western Blot analysis of various cells using Actin beta Polyclonal Antibody diluted at 1: 2000
Western Blot analysis of Hela cells using Actin beta Polyclonal Antibody diluted at 1: 2000
