PIK3R1 Monoclonal Antibody

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Home > Product > Antibody > PIK3R1 Monoclonal Antibody

PIK3R1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA4490

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human PIK3R1 (AA: 159-388) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

Uniprot accession

P27986:;

Molecular weight

83.6kDa

Gene ID

5295

Synonyms

p85;GRB1;p85-ALPHA

Research area

Signal Transduction

Target protein

PIK3R1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:100
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using PIK3R1 mAb against human PIK3R1 recombinant protein. (Expected MW is 53.4 kDa)

Immunofluorescence analysis of HeLa cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence analysis of HepG2 cells using PIK3R1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of NIH3T3 cells using PIK3R1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PIK3R1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIK3R1 mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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