CK5 Monoclonal Antibody

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Home > Product > Antibody > CK5 Monoclonal Antibody

CK5 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Monkey

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Monkey

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA2762

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CK5 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Uniprot accession

P13647:;

Molecular weight

62.3kDa

Gene ID

3852

Synonyms

KRT5;K5;DDD;EBS2;KRT5A

Research area

Others

Target protein

CK5

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using CK5 mAb against human CK5 (AA: 158-272) recombinant protein. (Expected MW is 33.3 kDa)

Western blot analysis using CK5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CK5 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using CK5 mouse mAb with DAB staining.

Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunofluorescence analysis of HeLa cells using CK5 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of HeLa cells using CK5 mouse mAb (green) and negative control (red).

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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