NEFL Monoclonal Antibody

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Home > Product > Antibody > NEFL Monoclonal Antibody

NEFL Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3400

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human NEFL expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y.

Uniprot accession

P07196:;

Molecular weight

62kDa

Gene ID

4747

Synonyms

NFL;NF-L;NF68;CMT1F;CMT2E

Research area

Neuroscience

Target protein

NEFL

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using NEFL mAb against human NEFL (AA: 422-543) recombinant protein. (Expected MW is 62 kDa)

Western blot analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.

Immunofluorescence analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemical analysis of paraffin-embedded lung cancer tissues using NEFL mouse mAb with DAB staining.

Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using NEFL mouse mAb with DAB staining.

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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