SKP1 Monoclonal Antibody

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Home > Product > Antibody > SKP1 Monoclonal Antibody

SKP1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA3199

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human SKP1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.

Uniprot accession

P63208:;

Molecular weight

19kDa

Gene ID

6500

Synonyms

OCP2;p19A;EMC19;SKP1A;OCP-II;TCEB1L

Research area

Cell Biology

Target protein

SKP1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using SKP1 mAb against human SKP1 (AA: 1-160) recombinant protein. (Expected MW is 50 kDa)

Western blot analysis using SKP1 mouse mAb against Hela (1), RAJI (2), Jurkat (3), MCF-7 (4), HepG2 (5), PC-12 (6) and Cos7 (7) cell lysate.

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using SKP1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SKP1 mouse mAb with DAB staining.

Flow cytometric analysis of Hela cells using SKP1 mouse mAb (green) and negative control (red).

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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