RAF1 Monoclonal Antibody

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Blood coagulation
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Home > Product > Antibody > RAF1 Monoclonal Antibody

RAF1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA3221

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human RAF1 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.

Uniprot accession

P04049:;

Molecular weight

73kDa

Gene ID

5894

Synonyms

NS5;CRAF;Raf-1;c-Raf

Research area

Signal Transduction

Target protein

RAF1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using RAF1 mAb against human RAF1 (AA: 198-407) recombinant protein. (Expected MW is 48.6 kDa)

Western blot analysis using RAF1 mouse mAb against HeLa (1), A431 (2), HepG (3), and SW620 (4)cell lysate.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using RAF1 mouse mAb with DAB staining.

Flow cytometric analysis of MCF-7 cells using RAF1 mouse mAb (green) and negative control (red).

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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