UBE2I Monoclonal Antibody

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Home > Product > Antibody > UBE2I Monoclonal Antibody

UBE2I Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Monkey

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Monkey

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA2463

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human UBE2I expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.

Uniprot accession

P63279:;

Molecular weight

18kDa

Gene ID

7329

Synonyms

P18;UBC9;C358B7.1

Research area

Epigenetics and nuclear signaling,Metabolism,Cell biology

Target protein

UBE2I

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using UBE2I mAb against human UBE2I (AA: 1-158) recombinant protein. (Expected MW is 45.3 kDa)

Western blot analysis using UBE2I mouse mAb against Hela (1), HepG2 (2), and Cos7 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded prostate tissues using UBE2I mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using UBE2I mouse mAb with DAB staining.

Immunofluorescence analysis of HepG2 cells using UBE2I mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of HepG2 cells using UBE2I mouse mAb (green) and negative control (red).

Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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