EIF4E Monoclonal Antibody

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Home > Product > Antibody > EIF4E Monoclonal Antibody

EIF4E Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3514

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human EIF4E expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regulators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Uniprot accession

P06730:;

Molecular weight

25kDa

Gene ID

1977

Synonyms

CBP;EIF4F;EIF4E1;EIF4EL1;MGC111573;EIF4E

Research area

Epigenetics and Nuclear Signaling

Target protein

EIF4E

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Western blot analysis using EIF4E mouse mAb against Hela (1), HEK293 (2) and K562 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded liver cancer (left) and submaxillary tumor (right) using EIF4E mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using EIF4E mouse mAb with DAB staining.

Immunofluorescence analysis of GC-7901 cells using EIF4E mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric analysis of Hela cells using EIF4E mouse mAb (green) and negative control (purple).

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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