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Home > Product > Antibody > CD44 Monoclonal Antibody

CD44 Monoclonal Antibody

CD44 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA3322

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD44 (628-699) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

CD44, also known as IN, LHRMIC4, CDW44, HCELL. It is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). This protein participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Uniprot accession

Molecular weight

82kDa

Gene ID

960

Synonyms

IN;LHR;MC56;MDU2;MDU3;MIC4;Pgp1;CDW44;CSPG8;HCELL

Research area

Immunology

Target protein

CD44

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
ICC 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Western blot analysis using CD44 mouse mAb against truncated Trx-CD44 recombinant protein (1) and GST-CD44 (aa628-699) recombinant protein (2).
Western blot analysis using CD44 mouse mAb against Hela (1) and HUVE-12(2) cell lysate.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissues, showing membrane localization with DAB staining using CD44 mouse mAb.
Confocal analysis of paraffin-embedded human lung cancer tissues using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Confocal Immunofluorescence analysis of PANC-1 cells using CD44 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Confocal Immunofluorescence analysis of methanol-fixed A431 (A), Hela (B), PANC-1 (C) and EC (D) cells using CD44 mouse mAb (green), showing membrane localization. Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of Hela cells using CD44 mouse mAb (right) and negative control (left).

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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