NANOG Monoclonal Antibody

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Blood coagulation
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Home > Product > Antibody > NANOG Monoclonal Antibody

NANOG Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,ICC

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,ICC

Product overview

Code

BT-MCA3280

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of NANOG (aa20-166) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

NANOG: Nanog homeobox. Entrez Protein NP_079141. Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression were detected by Northern analysis in the undifferentiated N-Tera embryonal carcinoma cell line.

Uniprot accession

Q9H9S0:;

Molecular weight

35kDa

Gene ID

79923

Synonyms

NANOG

Research area

Epigenetics and nuclear signaling,Developmental biology,Stem cells,Cancer

Target protein

NANOG

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
ELISA	1:10000

Images

Western blot analysis using NANOG mouse mAb against NTERA-2 cell lysate (2).

Confocal Immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using Nanog mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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