MDM4 Monoclonal Antibody

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Home > Product > Antibody > MDM4 Monoclonal Antibody

MDM4 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: IHC,ICC

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: IHC,ICC

Product overview

Code

BT-MCA3238

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MDM4 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

MDM4, encodes a 490-amino acid protein containing a RING finger domain and a putative nuclear localization signal. The MDM4 putative nuclear localization signal, which all Mdm proteins contain, is located in the C-terminal region of the protein. The mRNA is expressed at a high level in thymus and at lower levels in all other tissues tested. MDM4 protein produced by in vitro translation interacts with p53 via a binding domain located in the N-terminal region of the MDM4 protein. MDM4 shows significant structural similarity to p53-binding protein MDM2.

Uniprot accession

O15151:;

Molecular weight

55kDa

Gene ID

4194

Synonyms

HDMX;MDMX;MRP1;MDM4

Research area

Cell Biology

Target protein

MDM4

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
ELISA	1:10000

Images

Western blot analysis using MDM4 mouse mAb against Hela (1), A549 (2) and A431 (3) cell lysate.

Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDM4 mouse mAb.

Immunohistochemical analysis of paraffin-embedded human Tonsil tissues using MDM4 mouse mAb

Confocal Immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDM4 mouse mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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