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Home > Product > Antibody > HSP60 Monoclonal Antibody

HSP60 Monoclonal Antibody

HSP60 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat, Mouse, Monkey

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat,Mouse,Monkey

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4846

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human HSP60 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13. (provided by RefSeq)

Uniprot accession

Molecular weight

61kDa

Gene ID

3329

Synonyms

HLD4;CPN60;GROEL;HSP60;HSP65;SPG13;HSP-60;HuCHA60;HSPD1

Research area

Others

Target protein

HSP60

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
ICC 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using HSP60 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 mouse mAb with DAB staining.
Immunofluorescence analysis of 3T3-L1 cells using HSP60 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow cytometric analysis of Hela cells using HSP60 mouse mAb (green) and negative control (purple).
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Protocols

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Product size

Price

  • 100μL

    $180.00

  • 50μL

    $120.00

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Lead time: Within one week

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