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Product > Antibody > His Tag Monoclonal Antibody
His Tag Monoclonal Antibody
His Tag Monoclonal Antibody
Product Summary
Size:
50μL
Clonality: 6A8H8
Host: Mouse
Reactivity:Tag
Applications: WB,IP
Product Summary
Size:
50μL
Clonality: 6A8H8
Host: Mouse
Reactivity: Tag
Applications: WB,IP
Product overview
Code
BT-MCA4855
Host
Mouse
Isotype
Mouse IgG1
Size
50μL
Clonality
6A8H8
Immunogen
Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term.
Background
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.
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