His Tag Monoclonal Antibody

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OneStep ELISA kit
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Recombinant proteins

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Blood coagulation
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Home > Product > Antibody > His Tag Monoclonal Antibody

His Tag Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IP,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IP,IHC,ICC,FCM

Product overview

Code

BT-MCA4856

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.

Synonyms

6 His tag;HHHHHH tag

Research area

Others

Target protein

His Tag

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:100 - 1:1000
ICC	1:100 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000
IP	1:20

Images

Red: Control Antigen (100ng) Purple: Antigen (10ng), Green: Antigen (50ng) Blue: Antigen (100ng)

Western blot analysis using His tag mAb against recombinant protein. lane 1: (100 ng); lane 2: (50 ng); lane 3: (25 ng); lane 4: (10 ng); lane 5: (2.5 ng); (Expected MW is 50 kDa)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$180.00
50μL

$120.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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