PDGFA Monoclonal Antibody

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Home > Product > Antibody > PDGFA Monoclonal Antibody

PDGFA Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: ICC,FCM

Product overview

Code

BT-MCA2802

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human PDGFA (AA: 87-211) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants.

Uniprot accession

P04085:;

Molecular weight

24kDa

Gene ID

5154

Synonyms

PDGF1;PDGF-A

Research area

Cardiovascular

Target protein

PDGFA

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:50 - 1:200
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using PDGFA mAb against human PDGFA (AA: 87-211) recombinant protein. (Expected MW is 40.5 kDa)

Western blot analysis using PDGFA mAb against HEK293 (1) and PDGFA (AA: 87-211)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis of Hela cells using PDGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis of NIH/3T3 cells using PDGFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Jurkat cells using PDGFA mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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