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Home > Product > Antibody > HLA-DPA1 Monoclonal Antibody

HLA-DPA1 Monoclonal Antibody

HLA-DPA1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Rat

Applications: IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Rat

Applications: IHC,FCM

Product overview

Code

BT-MCA2293

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human HLA-DPA1 (AA: 29-209) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Uniprot accession

Molecular weight

29.3kDa

Gene ID

3113

Synonyms

DPA1;PLT1;HLADP;HLASB;DP(W3);DP(W4);HLA-DPA;HLA-DP1A;HLA-DPB1

Research area

Others

Target protein

HLA-DPA1

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using HLA-DPA1 mAb against human HLA-DPA1 (AA: 29-209) recombinant protein. (Expected MW is 23.7 kDa)
Western blot analysis using HLA-DPA1 mAb against HEK293-6e (1) and HLA-DPA1 (AA:29-209)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis of Hela cells using HLA-DPA1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HLA-DPA1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rat spleen tissues using HLA-DPA1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rabbit spleen tissues using HLA-DPA1 mouse mAb with DAB staining.

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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