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Home > Product > Antibody > NOTCH2 Monoclonal Antibody

NOTCH2 Monoclonal Antibody

NOTCH2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA2654

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human NOTCH2 (AA: extra 1391-1677) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Molecular weight

265.4kDa

Gene ID

4853

Synonyms

hN2;AGS2;HJCYS

Research area

Neuroscience

Target protein

NOTCH2

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
ICC 1:50 - 1:200
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using NOTCH2 mAb against human NOTCH2 (AA:extra 1391-1677) recombinant protein. (Expected MW is 34.8 kDa)
Western blot analysis using NOTCH2 mAb against HEK293-6e (1) and NOTCH2 (AA:extra 1391-1677)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis of Hela cells using NOTCH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Raji cells using NOTCH2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded renal carcinoma tissues using NOTCH2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NOTCH2 mouse mAb with DAB staining.

Protocols

Customer reviews and Q&As

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

Add to Cart

Lead time: Within one week

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