MYOD1 Monoclonal Antibody

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Home > Product > Antibody > MYOD1 Monoclonal Antibody

MYOD1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: ICC,FCM

Product overview

Code

BT-MCA4234

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MYOD1 (AA: 221-320) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.

Uniprot accession

P15172:;

Molecular weight

34.5kDa

Gene ID

4654

Synonyms

PUM;MYF3;MYOD;bHLHc1;MYODRIF

Research area

Epigenetics and Nuclear Signaling

Target protein

MYOD1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:50 - 1:200
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using MYOD1 mAb against human MYOD1 (AA: 221-320) recombinant protein. (Expected MW is 48 kDa)

Western blot analysis using MYOD1 mAb against HEK293-6e (1) and MYOD1 (AA: 221-320)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis of Hela cells using MYOD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using MYOD1 mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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