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Home > Product > Antibody > SH2B2 Monoclonal Antibody

SH2B2 Monoclonal Antibody

SH2B2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: IHC,FCM

Product overview

Code

BT-MCA4598

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human SH2B2 (AA: 497-676) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is expressed in B lymphocytes and contains pleckstrin homology and src homology 2 (SH2) domains. In Burkitt's lymphoma cell lines, it is tyrosine-phosphorylated in response to B cell receptor stimulation. Because it binds Shc independent of stimulation and Grb2 after stimulation, it appears to play a role in signal transduction from the receptor to the Shc/Grb2 pathway.

Uniprot accession

Molecular weight

67.7kDa

Gene ID

10603

Synonyms

APS

Research area

Others

Target protein

SH2B2

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using SH2B2 mAb against human SH2B2 (AA: 497-676) recombinant protein. (Expected MW is 21.8 kDa)
Western blot analysis using SH2B2 mAb against HEK293-6e (1) and SH2B2 (AA: 497-676)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis of HEK293 cells using SH2B2 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using SH2B2 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using SH2B2 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded colon cancer tissues using SH2B2 mouse mAb with DAB staining.

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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