H2AFX Monoclonal Antibody

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Blood coagulation
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Home > Product > Antibody > H2AFX Monoclonal Antibody

H2AFX Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3284

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human H2AFX expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.

Uniprot accession

P16104:;

Molecular weight

15.1kDa

Gene ID

3014

Synonyms

H2AX;H2A.X;H2A/X

Research area

Epigenetics and Nuclear Signaling

Target protein

H2AFX

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:50 - 1:200
FCM	1:200 - 1:400

Images

Western blot analysis using H2AFX mouse mAb against HEK293 (1), HepG2 (2), Jurkat (3),Hela (4), Raji (5), K562 (6) and HCT116 (7) cell lysate.

Immunofluorescence analysis of Hela cells using H2AFX mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using H2AFX mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using H2AFX mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded cerebellum tissues using H2AFX mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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