TOP1 Monoclonal Antibody

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Blood coagulation
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Epigenetics and nuclear signaling
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Home > Product > Antibody > TOP1 Monoclonal Antibody

TOP1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA4743

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human TOP1 expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Uniprot accession

P11387:;

Molecular weight

90.7kDa

Gene ID

7150

Synonyms

TOPI

Research area

Epigenetics and Nuclear Signaling

Target protein

TOP1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:100 - 1:400
FCM	1:200 - 1:400

Images

Western blot analysis using TOP1 mouse mAb against HUVEC (1), Jukat (2),MCF-7 (3),PC-12 (4),Hela (5),NIH/3T3 (6) and HCT116 (7) cell lysate.

Immunofluorescence analysis of Hela cells using TOP1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using TOP1 mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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