HNF1B Monoclonal Antibody

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Home > Product > Antibody > HNF1B Monoclonal Antibody

HNF1B Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Rat

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA2687

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human HNF1B (AA: 23-132) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the homeodomain-containing superfamily of transcription factors. The protein binds to DNA as either a homodimer, or a heterodimer with the related protein hepatocyte nuclear factor 1-alpha. The gene has been shown to function in nephron development, and regulates development of the embryonic pancreas. Mutations in this gene result in renal cysts and diabetes syndrome and noninsulin-dependent diabetes mellitus, and expression of this gene is altered in some types of cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

P35680:;

Molecular weight

61.3kDa

Gene ID

6928

Synonyms

T2D;FJHN;HNF2;LFB3;RCAD;TCF2;HPC11;LF-B3;MODY5;TCF-2;VHNF1;ADTKD3;HNF-1B;HNF1beta;HNF-1-beta

Research area

Epigenetics and Nuclear Signaling

Target protein

HNF1B

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:50 - 1:200
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using HNF1B mAb against human HNF1B (AA: 23-132) recombinant protein. (Expected MW is 38 kDa)

Western blot analysis using HNF1B mAb against HEK293-6e (1) and HNF1B (AA:23-132)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using HNF1B mouse mAb against Hela (1) cell lysate.

Immunofluorescence analysis of Hela cells using HNF1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of PC-3 cells using HNF1B mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded rat kidney tissues using HNF1B mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded colon tissues using HNF1B mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rabbit kidney tissues using HNF1B mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using HNF1B mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using HNF1B mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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