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Home > Product > Antibody > IL10RB Monoclonal Antibody

IL10RB Monoclonal Antibody

IL10RB Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: ICC,FCM

Product overview

Code

BT-MCA2315

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human IL10RB(AA: 20-220) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21.

Uniprot accession

Molecular weight

37kDa

Gene ID

3588

Synonyms

CRFB4;CRF2-4;D21S58;D21S66;CDW210B;IL-10R2

Research area

Immunology

Target protein

IL10RB

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
ICC 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using IL10RB mAb against human IL10RB (AA: 20-220) recombinant protein. (Expected MW is 49.5 kDa)
Western blot analysis using IL10RB mAb against HEK293-6e (1) and IL10RB (AA: 20-220)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis of Hela cells using IL10RB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of Jurkat cells using IL10RB mouse mAb (green) and negative control (red).

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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