POU5F1 Monoclonal Antibody

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Home > Product > Antibody > POU5F1 Monoclonal Antibody

POU5F1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: ICC,FCM

Product overview

Code

BT-MCA3745

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human POU5F1 (AA: 136-360) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.

Uniprot accession

Q01860:;

Molecular weight

38.5kDa

Gene ID

5460

Synonyms

OCT3;OCT4;OTF3;OTF4;OTF-3;Oct-3;Oct-4

Research area

Epigenetics and Nuclear Signaling

Target protein

POU5F1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:50 - 1:200
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using POU5F1 mAb against human POU5F1 (AA: 136-360) recombinant protein. (Expected MW is 65.2 kDa)

Western blot analysis using POU5F1 mAb against HEK293-6e (1) and POU5F1 (AA: 136-360）-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis of Hela cells using POU5F1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of K562 cells using POU5F1 mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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