IL1B Monoclonal Antibody

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Home > Product > Antibody > IL1B Monoclonal Antibody

IL1B Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:

Applications: ICC,FCM

Product overview

Code

BT-MCA4195

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human IL1B (AA: 117-269) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase2 by this cytokine in the central nervous system is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Molecular weight

30.7kDa

Gene ID

3553

Synonyms

IL-1;IL1F2;IL1beta;IL1-BETA

Research area

Immunology

Target protein

IL1B

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using IL1B mAb against human IL1B (AA: 117-269) recombinant protein. (Expected MW is 43.4 kDa)

Flow cytometric analysis of Jurkat cells using IL1B mouse mAb (green) and negative control (red).

Flow cytometric analysis of MOLT4 cells using IL1B mouse mAb (green) and negative control (red).

Flow cytometric analysis of THP-1 cells using IL1B mouse mAb (green) and negative control (red).

Immunofluorescence analysis of Hela cells using IL1B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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