HIF2a Monoclonal Antibody

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Home > Product > Antibody > HIF2a Monoclonal Antibody

HIF2a Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA4513

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a transcription factor involved in the induction of genes regulated by oxygen, which is induced as oxygen levels fall. The encoded protein contains a basic-helix-loop-helix domain protein dimerization domain as well as a domain found in proteins in signal transduction pathways which respond to oxygen levels. Mutations in this gene are associated with erythrocytosis familial type 4. [provided by RefSeq, Nov 2009]

Uniprot accession

Q99814:;

Molecular weight

96.5kDa

Gene ID

2034

Synonyms

HLF;MOP2;ECYT4;HIF2A;PASD2;bHLHe73

Research area

Others

Target protein

HIF2a

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using HIF2a mAb against human HIF2a (AA: 680-870) recombinant protein. (Expected MW is 23.2 kDa)

Immunofluorescence analysis of Hela cells using HIF2a mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using HIF2a mouse mAb (green) and negative control (red).

Flow cytometric analysis of HepG2 cells using HIF2a mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded baldder cancer tissues using HIF2a mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded kidney tissues using HIF2a mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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