ACADM Monoclonal Antibody

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Home > Product > Antibody > ACADM Monoclonal Antibody

ACADM Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA2502

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human ACADM (AA: 26-185) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

P11310:;

Molecular weight

46.6KDa

Gene ID

Synonyms

MCAD;ACAD1;MCADH

Research area

Signal Transduction,Metabolism

Target protein

ACADM

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using ACADM mAb against human ACADM (AA: 26-185) recombinant protein. (Expected MW is 20.5kDa)

Western blot analysis using ACADM mAb against HEK293-6e (1) and ACADM (AA: 26-185)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using ACADM mouse mAb against Hela (1), HepG2 (2),Jurkat (3),Raji (4) and K562 (5) cell lysate.

Immunofluorescence analysis of Hela cells using ACADM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of HL-60 cells using ACADM mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ACADM mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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