AHNAK Monoclonal Antibody

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Blood coagulation
Metabolism
Cancer
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Neuroscience
Cell biology
Signal transduction
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Epigenetics and nuclear signaling
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Home > Product > Antibody > AHNAK Monoclonal Antibody

AHNAK Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA4797

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human AHNAK (AA: 1-200) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a large (700 kDa) structural scaffold protein consisting of a central domain with 128 aa repeats. The encoded protein may play a role in such diverse processes as blood-brain barrier formation, cell structure and migration, cardiac calcium channel regulation, and tumor metastasis. A much shorter variant encoding a 17 kDa isoform exists for this gene, and the shorter isoform initiates a feedback loop that regulates alternative splicing of this gene.

Uniprot accession

Q09666:;

Molecular weight

63KDa

Gene ID

79026

Synonyms

PM227;AHNAKRS

Research area

Cancer,Signal transduction

Target protein

AHNAK

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using AHNAK mAb against human AHNAK (AA: 1-200) recombinant protein. (Expected MW is 25.2 kDa)

Western blot analysis using AHNAK mAb against HEK293-6e (1) and AHNAK (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis of Hela cells using AHNAK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using AHNAK mouse mAb (green) and negative control (red).

Flow cytometric analysis of HepG2 cells using AHNAK mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded Cervical cancer tissues using AHNAK mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using AHNAK mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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