HSPA9 Monoclonal Antibody

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Home > Product > Antibody > HSPA9 Monoclonal Antibody

HSPA9 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Human, Mouse, Monkey, Rat

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Human,Mouse,Monkey,Rat

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA2062

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human HSPA9 (AA: 480-679) expressed in mammalian.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.

Uniprot accession

P38646:;

Molecular weight

74KDa

Gene ID

3313

Synonyms

CSA;MOT;MOT2;SAAN;CRP40;EVPLS;GRP75;PBP74;GRP-75;HSPA9B;SIDBA4;MTHSP75;HEL-S-124m

Research area

Others

Target protein

HSPA9

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using HSPA9 mAb against human HSPA9 (AA: 480-679) recombinant protein. (Expected MW is 25.2 kDa)

Western blot analysis using HSPA9 mouse mAb against Jurkat (1), HepG2 (2), A431 (3), Hela (4), K562 (5), MCF-7 (6), C2C12 (7), A549 (8), PANC-1 (9), PC-12 (10), C6 (11), COS-7 (12)and NIH3T3 (13) cell lysate.

Immunofluorescence analysis of Hela cells using HSPA9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Jurkat cells using HSPA9 mouse mAb (green) and negative control (red).

Flow cytometric analysis of THP-1 cells using HSPA9 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using HSPA9 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using HSPA9 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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