CD220 Monoclonal Antibody

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Home > Product > Antibody > CD220 Monoclonal Antibody

CD220 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA2393

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD220 (AA: extra 413-624) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants.

Uniprot accession

P06213:;

Molecular weight

156kDa

Gene ID

3643

Synonyms

INSR;HHF5

Research area

Others

Target protein

CD220

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:50 - 1:200
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CD220 mAb against human CD220 (AA: extra 413-624) recombinant protein. (Expected MW is 27.8 kDa)

Western blot analysis using CD220 mAb against HEK293-6e (1) and CD220 (AA: extra 413-624)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using CD220 mouse mAb against HepG2 (1), HEK293 (2), Jurkat (3), Hela (4), THP-1 (5), NIH/3T3 (6), COS7 (7), and PC-3 (8) cell lysate.

Immunofluorescence analysis of Hela cells using CD220 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of HL-60 cells using CD220 mouse mAb (green) and negative control (red).

Flow cytometric analysis of THP-1 cells using CD220 mouse mAb (green) and negative control (red).

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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