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Home > Product > Antibody > B4GALNT1 Monoclonal Antibody

B4GALNT1 Monoclonal Antibody

B4GALNT1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA4705

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human B4GALNT1 (AA: 26-225) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1,4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

Molecular weight

58.9kDa

Gene ID

2583

Synonyms

GALGT;SPG26;GALNACT;GalNAc-T

Research area

Signal Transduction,Metabolism

Target protein

B4GALNT1

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using B4GALNT1 mAb against human B4GALNT1 (AA: 26-225) recombinant protein. (Expected MW is 24.4 kDa)
Western blot analysis using B4GALNT1 mAb against HEK293-6e (1) and human B4GALNT1 (AA: 26-225)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western blot analysis using B4GALNT1 mouse mAb against Hela (1), Jurkat (2), HepG2 (3), k562 (4), COS-7 (5), and NIH/3T3 (6) cell lysate.
Flow cytometric analysis of Hela cells using B4GALNT1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded brain tissues using B4GALNT1 mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using B4GALNT1 mouse mAb with DAB staining.

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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