RHOA Monoclonal Antibody

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > Antibody > RHOA Monoclonal Antibody

RHOA Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4051

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human RHOA (AA: 1-190) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Uniprot accession

P61586:;

Molecular weight

22kDa

Gene ID

387

Synonyms

ARHA;ARH12;RHO12;EDFAOB;RHOH12

Research area

Signal Transduction

Target protein

RHOA

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using RHOA mAb against human RHOA (AA: 1-190) recombinant protein.

Western blot analysis using RHOA mAb against HEK293 (1) and RHOA (AA: 1-190)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using RHOA mouse mAb against Jurlat (1), MCF-7 (2),A431 (3), and Hela (4) cell lysate.

Immunofluorescence analysis of Hela cells using RHOA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using RHOA mouse mAb (green) and negative control (red).

Flow cytometric analysis of HepG2 cells using RHOA mouse mAb (green) and negative control (red).

Flow cytometric analysis of Jurkat cells using RHOA mouse mAb (green) and negative control (red).

Flow cytometric analysis of K562 cells using RHOA mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded gastric cancer tissues using RHOA mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded endometrial carcinoma tissues using RHOA mouse mAb with DAB staining.

Protocols

> Protocol Booklet

Customer reviews and Q&As

No reviews

Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

We are continuously updating our Antibody catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.