ETFA Monoclonal Antibody

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Home > Product > Antibody > ETFA Monoclonal Antibody

ETFA Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA4023

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human ETFA (AA: 134-333) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot accession

P13804:;

Molecular weight

35kDa

Gene ID

2108

Synonyms

EMA;GA2;MADD

Research area

Others

Target protein

ETFA

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using ETFA mAb against human ETFA (AA: 134-333) recombinant protein. (Expected MW is 24 kDa)

Western blot analysis using ETFA mAb against HEK293 (1) and ETFA (AA:134-333)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using ETFA mouse mAb against .HepG2 (1), A431 (2), Hek293 (3),Hela (4)and MCF-7 (5) cell lysate.

Immunofluorescence analysis of Hela cells using ETFA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using ETFA mouse mAb (green) and negative control (red).

Flow cytometric analysis of HepG2 cells using ETFA mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using ETFA mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using ETFA mouse mAb with DAB staining.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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