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Home > Product > Antibody > ATP6AP1 Monoclonal Antibody

ATP6AP1 Monoclonal Antibody

ATP6AP1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,FCM

Product overview

Code

BT-MCA3011

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development.

Uniprot accession

Molecular weight

52kDa

Gene ID

537

Synonyms

16A;CF2;Ac45;XAP3;XAP-3;ATP6S1;VATPS1;ATP6IP1

Research area

Tags & cell markers

Target protein

ATP6AP1

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using ATP6AP1 mAb against human ATP6AP1 (AA: 51-151) recombinant protein. (Expected MW is 37kDa)
Western blot analysis using ATP6AP1 mAb against HEK293-6e (1) and human ATP6AP1 (AA: 51-151)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western blot analysis using ATP6AP1 mouse mAb against PC-3 (1), and Raw264.7 (2) cell lysate.
Flow cytometric analysis of Hela cells using ATP6AP1 mouse mAb (green) and negative control (red).

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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