CD200 Monoclonal Antibody

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Home > Product > Antibody > CD200 Monoclonal Antibody

CD200 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: ICC,FCM

Product overview

Code

BT-MCA2730

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human CD200 (AA:Extra(31-232)) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms.

Uniprot accession

P41217:;

Molecular weight

31.3kDa

Gene ID

4345

Synonyms

MRC;MOX1;MOX2;OX-2

Research area

Immunology

Target protein

CD200

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using CD200 mAb against human CD200 (AA:Extra(31-232)) recombinant protein. (Expected MW is 25 kDa)

Western blot analysis using CD200 mAb against HEK293-6e (1) and human CD200 (AA:Extra(31-232))-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of Raji cells using CD200 mouse mAb (green) and negative control (red).

Flow cytometric analysis of K562 cells using CD200 mouse mAb (green) and negative control (red).

Flow cytometric analysis of THP-1 cells using CD200 mouse mAb (green) and negative control (red).

Immunofluorescence analysis of Hela cells using CD200 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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