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Home > Product > Antibody > ATP5F1A Monoclonal Antibody

ATP5F1A Monoclonal Antibody

ATP5F1A Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,FCM

Product overview

Code

BT-MCA3588

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human ATP5F1A (AA: 44-220) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Uniprot accession

Molecular weight

59.8kDa

Gene ID

498

Synonyms

OMR;ORM;ATPM;MOM2;ATP5A;hATP1;ATP5A1;MC5DN4;ATP5AL2;COXPD22;HEL-S-123m

Research area

Others

Target protein

ATP5F1A

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
IHC-p 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using ATP5F1A mAb against human ATP5F1A (AA: 44-220) recombinant protein. (Expected MW is 44.7 kDa)
Western blot analysis using ATP5F1A mAb against HEK293-6e (1) and ATP5F1A (AA: 44-220)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western blot analysis using ATP5F1A mouse mAb against COS7 (1), NIH/3T3 (2), mouse heart (3), rat heart (4), HCT116 (5), Hela (6), and HepG2 (7) cell lysate.
Flow cytometric analysis of Jurkat cells using ATP5F1A mouse mAb (green) and negative control (red).
Immunohistochemical analysis of paraffin-embedded lung cancer tissues using ATP5F1A mouse mAb with DAB staining.
Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using ATP5F1A mouse mAb with DAB staining.

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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