MSH2 Monoclonal Antibody

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Home > Product > Antibody > MSH2 Monoclonal Antibody

MSH2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA2299

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human MSH2 (AA: (2-151) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.

Uniprot accession

P43246:;

Molecular weight

104.7kDa

Gene ID

4436

Synonyms

FCC1;COCA1;HNPCC;LCFS2;hMSH2;HNPCC1;MMRCS2

Research area

Epigenetics and Nuclear Signaling

Target protein

MSH2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using MSH2 mAb against human MSH2 (AA: (2-151) recombinant protein. (Expected MW is 19.6kDa)

Western blot analysis using MSH2 mAb against HEK293-6e (1) and MSH2 (AA:(2-151)-hIgGFc transfected HEK293-6e (2) cell lysate.

Flow cytometric analysis of Hela cells using MSH2 mouse mAb (green) and negative control (red).

Flow cytometric analysis of HepG2 cells using MSH2 mouse mAb (green) and negative control (red).

Western blot analysis using MSH2 mouse mAb against Hela (1), K562 (2), A549 (3), A431 (4), MCF-7 (5), DU145 (6), PC-3 (7),Raji (8), SW480 (9), COS-7 (10), NIH/3T3 (11), and PC-12 (12) cell lysate.

Immunofluorescence analysis of cells using MSH2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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