C5AR2 Monoclonal Antibody

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Home > Product > Antibody > C5AR2 Monoclonal Antibody

C5AR2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: IHC,ICC,FCM

Product overview

Code

BT-MCA3024

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human C5AR2 (AA: extra mix) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

This gene encodes a G-protein coupled receptor 1 family member involved in the complement system of the innate immune response. Unlike classical G-protein coupled receptors, the encoded protein does not associate with intracellular G-proteins. It may instead modulate signal transduction through the beta-arrestin pathway, and may alternatively act as a decoy receptor. This gene may be involved in coronary artery disease and in the pathogenesis of sepsis. Alternative splicing results in multiple transcript variants.

Uniprot accession

Q9P296:;

Molecular weight

36kDa

Gene ID

27202

Synonyms

C5L2;GPF77;GPR77

Research area

Others

Target protein

C5AR2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using C5AR2 mAb against human C5AR2 (AA: extra mix) recombinant protein. (Expected MW is 25 kDa)

Western blot analysis using C5AR2 mAb against HEK293-6e (1) and C5AR2 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.

Immunofluorescence analysis of Hela cells using C5AR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Molt4 cells using C5AR2 mouse mAb (green) and negative control (red).

Flow cytometric analysis of RAW24.7 cells using C5AR2 mouse mAb (green) and negative control (red).

Flow cytometric analysis of HL-60 cells using C5AR2 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using C5AR2 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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