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Home > Product > Antibody > ARG1 Monoclonal Antibody

ARG1 Monoclonal Antibody

ARG1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,ICC,FCM

Product overview

Code

BT-MCA2939

Host

Mouse

Isotype

Mouse IgG2b

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human ARG1 (AA:1-322) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot accession

Molecular weight

37 kDa

Gene ID

383

Synonyms

arginase 1

Research area

Signal Transduction,Metabolism

Target protein

ARG1

Recommended dilution

Application Dilution
WB 1:500 - 1:2000
ICC 1:200 - 1:1000
FCM 1:200 - 1:400
ELISA 1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western blot analysis using ARG1 mAb against human ARG1 (AA:1-322) recombinant protein. (Expected MW is 38.6kDa)
Western blot analysis using ARG1 mAb against HEK293-6e (1) and ARG1 (AA:1-322)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis of Hela cells using ARG1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis of BEL-7402 cells using ARG1 mouse mAb (green) and negative control (red).
Flow cytometric analysis of HepG2 cells using ARG1 mouse mAb (green) and negative control (red).

Protocols

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Product size

Price

  • 100μL

    $320.00

  • 50μL

    $220.00

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Lead time: Within one week

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