HDAC1 Monoclonal Antibody

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Home > Product > Antibody > HDAC1 Monoclonal Antibody

HDAC1 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: WB,IHC,ICC,FCM

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: WB,IHC,ICC,FCM

Product overview

Code

BT-MCA3625

Host

Mouse

Isotype

Mouse IgG1

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human HDAC1 (AA: 321-482) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Uniprot accession

Q13547:;

Molecular weight

55.1kDa

Gene ID

3065

Synonyms

HD1;RPD3;KDAC1;GON-10;RPD3L1

Research area

Epigenetics and Nuclear Signaling

Target protein

HDAC1

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
FCM	1:200 - 1:400
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using HDAC1 mAb against human HDAC1 (AA: 321-482) recombinant protein. (Expected MW is 44.6 kDa)

Western blot analysis using HDAC1 mAb against HEK293-6e (1) and HDAC1 (AA: 321-482)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using HDAC1 mouse mAb against Hela (1), Raw264.7 (2), K562 (3), and Jurkat (4) cell lysate.

Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis of Hela cells using HDAC1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis of Hela cells using HDAC1 mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded colon tissues using HDAC1 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded colon cancer tissues using HDAC1 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

Customer reviews and Q&As

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

Custom quote | Bulk request

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