KRT2 Monoclonal Antibody

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Home > Product > Antibody > KRT2 Monoclonal Antibody

KRT2 Monoclonal Antibody

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity:Others

Applications: IHC,ICC

Product Summary

Size: 100μL,50μL

Clonality: Monoclonal

Host: Mouse

Reactivity: Others

Applications: IHC,ICC

Product overview

Code

BT-MCA2205

Host

Mouse

Isotype

Mouse IgG2a

Size

100μL,50μL

Clonality

Monoclonal

Immunogen

Purified recombinant fragment of human KRT2 (AA: 177-490) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

Store at 4°C short term. Aliquot and store at -20°C long term.

Background

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in this gene have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.

Uniprot accession

P35908:;

Molecular weight

65.4kDa

Gene ID

3849

Synonyms

K2e;KRTE;CK-2e;KRT2A;KRT2E

Research area

Others

Target protein

KRT2

Recommended dilution

Application	Dilution
WB	1:500 - 1:2000
IHC-p	1:200 - 1:1000
ICC	1:200 - 1:1000
ELISA	1:10000

Images

Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western blot analysis using KRT2 mAb against human KRT2 (AA: 177-490) recombinant protein. (Expected MW is 52.4 kDa)

Western blot analysis using KRT2 mAb against HEK293-6e (1) and KRT2 (AA: 177-490)-hIgGFc transfected HEK293-6e (2) cell lysate.

Western blot analysis using KRT2 mouse mAb against A431 (1) cell lysate.

Immunofluorescence analysis of Hela cells using KRT2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using KRT2 mouse mAb with DAB staining.

Immunohistochemical analysis of paraffin-embedded breast cancer tissues using KRT2 mouse mAb with DAB staining.

Protocols

> Protocol Booklet

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Datasheet

Product size

Price

100μL

$320.00
50μL

$220.00

Add to Cart

Lead time: Within one week

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